recombinant mil-6 Search Results


recombinant interleukin il 6  (InvivoGen)
93
InvivoGen recombinant interleukin il 6
Radiation induces astrocyte senescence. (A) Representative image and (B) quantification of radiation-induced DNA damage identified by immunolabeling of double-stranded DNA breaks by 53BP1 and γH2AX in primary human astrocytes 3 days after radiation exposure (2 Gy). (C) Representative images of SA-β-gal staining in human astrocytes on day 3 after exposure to radiation (2 Gy). (D) Quantitative summary of the percent of astrocytes with SA-β-gal staining from 2 to 7 days after radiation (2 Gy). (E) Production of SASP-associated <t>cytokine</t> mRNAs <t>(IL-1β,</t> <t>IL-6,</t> and IL-8 mRNA) and (F) neurotrophic factor mRNAs (nerve growth factor [NGF], glial cell–derived neurotrophic factor [GDNF], insulin-like growth factor-1 [IGF-1]) in irradiated or sham-treated primary human astrocytes measured by qRT-PCR (Taqman). Representative images of (G) p16INK4A and (H) p21WAF1 immunolabeling in irradiated GFAP-positive human astrocytes on day 6 following exposure to radiation (2 Gy). (I) Quantitation of p16INK4A and p21WAF1 immunoreactivity in irradiated (2 Gy) and sham-treated primary human astrocytes on day 6. NS indicates P > 0.05, *P ≤ 0.05, **P ≤ 0.01, and ***P ≤ 0.001 by unpaired 2-tailed Student’s t-test. Scale = 25 μm.
Recombinant Interleukin Il 6, supplied by InvivoGen, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+mil-6/Recombinant+mouse+IL-6/pmc06422440-85-4-7
Average 93 stars, based on 1 article reviews
recombinant interleukin il 6 - by Bioz Stars, 2026-11
93/100 stars
  Buy from Supplier
recombinant cytokines and antibodies mil-6 and mil-12  (Biozol Diagnostica Vertrieb GmbH)
90
Biozol Diagnostica Vertrieb GmbH recombinant cytokines and antibodies mil-6 and mil-12
Radiation induces astrocyte senescence. (A) Representative image and (B) quantification of radiation-induced DNA damage identified by immunolabeling of double-stranded DNA breaks by 53BP1 and γH2AX in primary human astrocytes 3 days after radiation exposure (2 Gy). (C) Representative images of SA-β-gal staining in human astrocytes on day 3 after exposure to radiation (2 Gy). (D) Quantitative summary of the percent of astrocytes with SA-β-gal staining from 2 to 7 days after radiation (2 Gy). (E) Production of SASP-associated <t>cytokine</t> mRNAs <t>(IL-1β,</t> <t>IL-6,</t> and IL-8 mRNA) and (F) neurotrophic factor mRNAs (nerve growth factor [NGF], glial cell–derived neurotrophic factor [GDNF], insulin-like growth factor-1 [IGF-1]) in irradiated or sham-treated primary human astrocytes measured by qRT-PCR (Taqman). Representative images of (G) p16INK4A and (H) p21WAF1 immunolabeling in irradiated GFAP-positive human astrocytes on day 6 following exposure to radiation (2 Gy). (I) Quantitation of p16INK4A and p21WAF1 immunoreactivity in irradiated (2 Gy) and sham-treated primary human astrocytes on day 6. NS indicates P > 0.05, *P ≤ 0.05, **P ≤ 0.01, and ***P ≤ 0.001 by unpaired 2-tailed Student’s t-test. Scale = 25 μm.
Recombinant Cytokines And Antibodies Mil 6 And Mil 12, supplied by Biozol Diagnostica Vertrieb GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+mil-6/recombinant+cytokines+and+antibodies+mil+6+and+mil+12/pmc09304956-37-8-17
Average 90 stars, based on 1 article reviews
recombinant cytokines and antibodies mil-6 and mil-12 - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier
10 ng/ml recombinant mil-6  (Becton Dickinson)
90
Becton Dickinson 10 ng/ml recombinant mil-6
Radiation induces astrocyte senescence. (A) Representative image and (B) quantification of radiation-induced DNA damage identified by immunolabeling of double-stranded DNA breaks by 53BP1 and γH2AX in primary human astrocytes 3 days after radiation exposure (2 Gy). (C) Representative images of SA-β-gal staining in human astrocytes on day 3 after exposure to radiation (2 Gy). (D) Quantitative summary of the percent of astrocytes with SA-β-gal staining from 2 to 7 days after radiation (2 Gy). (E) Production of SASP-associated <t>cytokine</t> mRNAs <t>(IL-1β,</t> <t>IL-6,</t> and IL-8 mRNA) and (F) neurotrophic factor mRNAs (nerve growth factor [NGF], glial cell–derived neurotrophic factor [GDNF], insulin-like growth factor-1 [IGF-1]) in irradiated or sham-treated primary human astrocytes measured by qRT-PCR (Taqman). Representative images of (G) p16INK4A and (H) p21WAF1 immunolabeling in irradiated GFAP-positive human astrocytes on day 6 following exposure to radiation (2 Gy). (I) Quantitation of p16INK4A and p21WAF1 immunoreactivity in irradiated (2 Gy) and sham-treated primary human astrocytes on day 6. NS indicates P > 0.05, *P ≤ 0.05, **P ≤ 0.01, and ***P ≤ 0.001 by unpaired 2-tailed Student’s t-test. Scale = 25 μm.
10 Ng/Ml Recombinant Mil 6, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+mil-6/10+ng+ml+recombinant+mil+6/pm15507530-72-91-93
Average 90 stars, based on 1 article reviews
10 ng/ml recombinant mil-6 - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier

Image Search Results


Radiation induces astrocyte senescence. (A) Representative image and (B) quantification of radiation-induced DNA damage identified by immunolabeling of double-stranded DNA breaks by 53BP1 and γH2AX in primary human astrocytes 3 days after radiation exposure (2 Gy). (C) Representative images of SA-β-gal staining in human astrocytes on day 3 after exposure to radiation (2 Gy). (D) Quantitative summary of the percent of astrocytes with SA-β-gal staining from 2 to 7 days after radiation (2 Gy). (E) Production of SASP-associated cytokine mRNAs (IL-1β, IL-6, and IL-8 mRNA) and (F) neurotrophic factor mRNAs (nerve growth factor [NGF], glial cell–derived neurotrophic factor [GDNF], insulin-like growth factor-1 [IGF-1]) in irradiated or sham-treated primary human astrocytes measured by qRT-PCR (Taqman). Representative images of (G) p16INK4A and (H) p21WAF1 immunolabeling in irradiated GFAP-positive human astrocytes on day 6 following exposure to radiation (2 Gy). (I) Quantitation of p16INK4A and p21WAF1 immunoreactivity in irradiated (2 Gy) and sham-treated primary human astrocytes on day 6. NS indicates P > 0.05, *P ≤ 0.05, **P ≤ 0.01, and ***P ≤ 0.001 by unpaired 2-tailed Student’s t-test. Scale = 25 μm.

Journal: Neuro-Oncology

Article Title: Radiation-induced astrocyte senescence is rescued by Δ133p53

doi: 10.1093/neuonc/noz001

Figure Lengend Snippet: Radiation induces astrocyte senescence. (A) Representative image and (B) quantification of radiation-induced DNA damage identified by immunolabeling of double-stranded DNA breaks by 53BP1 and γH2AX in primary human astrocytes 3 days after radiation exposure (2 Gy). (C) Representative images of SA-β-gal staining in human astrocytes on day 3 after exposure to radiation (2 Gy). (D) Quantitative summary of the percent of astrocytes with SA-β-gal staining from 2 to 7 days after radiation (2 Gy). (E) Production of SASP-associated cytokine mRNAs (IL-1β, IL-6, and IL-8 mRNA) and (F) neurotrophic factor mRNAs (nerve growth factor [NGF], glial cell–derived neurotrophic factor [GDNF], insulin-like growth factor-1 [IGF-1]) in irradiated or sham-treated primary human astrocytes measured by qRT-PCR (Taqman). Representative images of (G) p16INK4A and (H) p21WAF1 immunolabeling in irradiated GFAP-positive human astrocytes on day 6 following exposure to radiation (2 Gy). (I) Quantitation of p16INK4A and p21WAF1 immunoreactivity in irradiated (2 Gy) and sham-treated primary human astrocytes on day 6. NS indicates P > 0.05, *P ≤ 0.05, **P ≤ 0.01, and ***P ≤ 0.001 by unpaired 2-tailed Student’s t-test. Scale = 25 μm.

Article Snippet: IL-6 Treatment Where indicated, recombinant interleukin (IL)-6 (InvivoGen) was incubated with iPSC-derived neural stem cells (NSCs) or mature neurons for 24 hours at a concentration of 5 ng/mL.

Techniques: Immunolabeling, Staining, Derivative Assay, Irradiation, Quantitative RT-PCR, Quantitation Assay

Δ133p53 regulates radiation-induced, astrocyte-mediated neurotoxicity. (A–B) Immunopositivity of cleaved caspase 3 in mature neurons and (C) viability of neural stem cells following 24-hour IL-6 exposure (5 ng/mL). (D) IL-6 mRNA production in sham and irradiated astrocytes transduced with either Δ133p53 (P = 0.389) or the control vector on day 3 and examined on day 6 by qRT-PCR (Taqman). (E) IL-6 protein secreted by astrocytes transduced prior to radiation and examined by enzyme-linked immunosorbent assay, (F) IGF-1 mRNA expression in irradiated astrocytes transduced with pLOC, or Δ133p53 vector prior to radiation exposure (P = 0.015) and examined by qRT-PCR (Taqman). NS indicates P > 0.05, *P ≤ 0.05, **P ≤ 0.01, and ***P ≤ 0.001 by unpaired 2-tailed Student’s t-test. Scale = 25 μm.

Journal: Neuro-Oncology

Article Title: Radiation-induced astrocyte senescence is rescued by Δ133p53

doi: 10.1093/neuonc/noz001

Figure Lengend Snippet: Δ133p53 regulates radiation-induced, astrocyte-mediated neurotoxicity. (A–B) Immunopositivity of cleaved caspase 3 in mature neurons and (C) viability of neural stem cells following 24-hour IL-6 exposure (5 ng/mL). (D) IL-6 mRNA production in sham and irradiated astrocytes transduced with either Δ133p53 (P = 0.389) or the control vector on day 3 and examined on day 6 by qRT-PCR (Taqman). (E) IL-6 protein secreted by astrocytes transduced prior to radiation and examined by enzyme-linked immunosorbent assay, (F) IGF-1 mRNA expression in irradiated astrocytes transduced with pLOC, or Δ133p53 vector prior to radiation exposure (P = 0.015) and examined by qRT-PCR (Taqman). NS indicates P > 0.05, *P ≤ 0.05, **P ≤ 0.01, and ***P ≤ 0.001 by unpaired 2-tailed Student’s t-test. Scale = 25 μm.

Article Snippet: IL-6 Treatment Where indicated, recombinant interleukin (IL)-6 (InvivoGen) was incubated with iPSC-derived neural stem cells (NSCs) or mature neurons for 24 hours at a concentration of 5 ng/mL.

Techniques: Irradiation, Transduction, Plasmid Preparation, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Expressing